Part:BBa_K4998022

LuxR gene

Usage and Biology

This part consists of the LuxR gene, which expresses a 251 amino acid protein. LuxR is a gene that takes part in the Lux operon since the protein it expresses operates as a regulatory agent, originating from Vibrio fischeri. The protein is expressed constantly and has the ability to bind to AHL (N-Acyl homoserine lactone) thus taking part in quorum sensing systems. [1] In our project, Euphoresis, we transfer the LuxR gene in a plasmid vector to a cyanobacterium strain, Nostoc oryzae TAU-MAC 2710. The protein that is produced binds to AHL, a signal molecule that in our quorum sensing system is expressed be a strain of B.subtilis, and the product acts as a transcription regulator of an antitoxin gene. The antitoxin that is expressed creates clumps with a constitutively expressed toxin and keeps the cyanobacterium alive. With the death of the B.subtilis strain, AHL production is stopped and the kill-switch mechanism of the cyanobacterium is activated.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 349
  

References

[1] Kokarakis, E. J., Rillema, R., Ducat, D. C., & Sakkos, J. K. (2022). Developing cyanobacterial quorum sensing toolkits: Toward interspecies coordination in mixed autotroph/heterotroph communities. ACS Synthetic Biology, 12(1), 265–276. https://doi.org/10.1021/acssynbio.2c00527